Abnormal gene expression in regular and aggregated somatic cell nuclear transfer placentas
نویسندگان
چکیده
منابع مشابه
Abnormal gene expression in regular and aggregated somatic cell nuclear transfer placentas
BACKGROUND Placental defects in somatic cell nuclear transfer (SCNT) are a major cause of complications during pregnancy. One of the most critical factors for the success of SCNT is the successful epigenetic reprogramming of donor cells. Recently, it was reported that the placental weight in mice cloned with the aggregated SCNT method was significantly reduced. Here, we examine the profile of a...
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Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
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This study was conducted to investigate the expression of three genes related to early embryonic development in bovine transgenic cloned embryos. To accomplish this, development of bovine transgenic somatic cell nuclear transfer (SCNT) embryos was compared with non-transgenic embryos. Next, mRNA transcription of three specific genes (DNMT1, Hsp 70.1, and Mash2) related to early embryo developme...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2017
ISSN: 1472-6750
DOI: 10.1186/s12896-017-0355-4